SUNNY


MORE PICTURES OF SUNNY

SUNNY’S GENETICS

E LOCUS
In PWDs, the E locus covers the spectrum of white, cream, yellow, apricot, and red.
GENE
MC1R – controls the production of pigment in melanocytes (pigment cells)
GENOTYPE:
e/e
The dominant E allele allows for production of eumelanin (black pigment), while the recessive e allele results in conversion of all eumelanin in the coat to phaeomelanin(red pigment), though the “ee” genotype varies in expression between different breeds and may appear anywhere from yellow to orange, tan, and red.
PHENOTYPE
Sunny’s “white” or rather cream phenotype required two copies of the dysfunctional, recessive allele of the MC1R extension gene. Chances of attaining this coat color were very slim.

The alternate “white” coat is the extreme piebald spotting, which takes on a snowy white appearance, but is lethal if bred back to another of the same genotype. Thus, Sunny’s ee genotype is “preferred”, as ee’s, when bred together, produce no problems linked with this gene in the litter.

EM LOCUS
Melanistic black mask
GENE
Melanocortin 1 receptor gene
GENOTYPE:
Em/E
Sunny carries the dominant Em (M264V) allele, which causes melanocyte-stimulating hormone to bind and activate melanocortin 1 receptors located on melanocytes aroundthe area of the mask. Melanocytes in this region produce eumelanin
PHENOTYPE
Because Sunny is e/e at the E locus, eumelanin is NOT produced and therefore, no mask is
visible
B LOCUS
GENE
TYRP1
GENOTYPE:
B/B
Full Color, does not carry brown. The TYRP1 gene codes for Tyrosinase, an enzyme that plays a role in synthesis of eumelanin pigment.

Because TYRP 1 protein is associated only with eumelanin production, a mutation in the B locus (recessive b allele) only has an effect on coat color of dogs that are EE or Ee at the E Locus.

PHENOTYPE

Full color
K LOCUS
GENE

CBD103, “Beta-defensin”
GENOTYPE:
K/K
Sunny is homozygous for the dominant K allele. Two recessive alleles are needed to express a brindled pattern or agouti.
PHENOTYPE
Because sunny is e/e at the E locus, eumelanin is not produced and therefore, no mask is visible.
D LOCUS
GENE
MLPH
The “dilute” MLPH gene encodes for the protein melanophilin, which transports and fixesmelanin-containing cells.
GENOTYPE:
D/D
Mutation at this locus (recessive d allele) affects eumelanin and phaeomelanin
pigment production, which alters the intensity of coat pigment.
PHENOTYPE
Sunny is DD, full colored.